Inhibition of lymphangiogenesis and lymphatic drainage via VEGFR-3 blockade increases the severity of inflammation in chronic inflammatory arthritis
Identifieur interne : 006236 ( Main/Exploration ); précédent : 006235; suivant : 006237Inhibition of lymphangiogenesis and lymphatic drainage via VEGFR-3 blockade increases the severity of inflammation in chronic inflammatory arthritis
Auteurs : Ruolin Guo [États-Unis] ; Quan Zhou [États-Unis] ; Steven T. Proulx [États-Unis] ; Ronald Wood [États-Unis] ; Rui-Cheng Ji [Japon] ; Christopher T. Ritchlin [États-Unis] ; Bronislaw Pytowski [États-Unis] ; Zhenping Zhu [États-Unis] ; Yong-Jun Wang [République populaire de Chine] ; Edward M. Schwarz [États-Unis] ; Lianping Xing [États-Unis]Source :
- Arthritis and rheumatism [ 0004-3591 ] ; 2009.
Abstract
Investigation of the effect of lymphatic inhibition on joint and draining lymph node pathology during the course of arthritis progression in mice.
TNF transgenic (TNF-Tg) mice were used as a model of chronic inflammatory arthritis. Mice received contrast enhanced MRI to obtain ankle and knee joint synovial volumes and draining popliteal lymph node (PLN) volumes before and 8 weeks after treatment with VEGFR-3 or VEGFR-2 neutralizing antibodies, or isotype IgG. The animals were subjected to near-infrared lymphatic imaging to determine the effect of VEGFR-3 neutralization on lymph transport from paws to draining PLNs prior to sacrifice. Lymphatic vessel formation and morphology of joints and PLNs were examined by histology, immunohistochemistry, and RT-PCR.
Compared to IgG treatment, VEGFR-3 neutralizing antibody treatment significantly decreased the size of PLNs, the number of lymphatic vessels in joints and PLNs, the lymphatic drainage from paws to PLNs, and the number of VEGF-C expressing CD11b+ myeloid cells in PLNs. However, it increased the synovial volumes and inflammatory area in ankle and knee joints. VEGFR-2 neutralizing antibody, in contrast, inhibited both lymphangiogenesis and joint inflammation.
Lymphangiogenesis and lymphatic drainage are reciprocally related to the severity of joint lesions during the development of chronic arthritis. Lymphatic drainage plays a beneficial role in controlling the progression of chronic inflammation.
Url:
DOI: 10.1002/art.24764
PubMed: 19714652
PubMed Central: 2810533
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 002761
- to stream Pmc, to step Curation: 002760
- to stream Pmc, to step Checkpoint: 003703
- to stream Ncbi, to step Merge: 003583
- to stream Ncbi, to step Curation: 003583
- to stream Ncbi, to step Checkpoint: 003583
- to stream Main, to step Merge: 006311
- to stream Main, to step Curation: 006236
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of lymphangiogenesis and lymphatic drainage via VEGFR-3 blockade increases the severity of inflammation in chronic inflammatory arthritis</title><author><name sortKey="Guo, Ruolin" sort="Guo, Ruolin" uniqKey="Guo R" first="Ruolin" last="Guo">Ruolin Guo</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Quan" sort="Zhou, Quan" uniqKey="Zhou Q" first="Quan" last="Zhou">Quan Zhou</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Proulx, Steven T" sort="Proulx, Steven T" uniqKey="Proulx S" first="Steven T." last="Proulx">Steven T. Proulx</name><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Wood, Ronald" sort="Wood, Ronald" uniqKey="Wood R" first="Ronald" last="Wood">Ronald Wood</name><affiliation wicri:level="1"><nlm:aff id="A3"> Department of Urology, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Urology, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Ji, Rui Cheng" sort="Ji, Rui Cheng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name><affiliation wicri:level="1"><nlm:aff id="A4"> Department of Anatomy, Biology and Medicine, Oita University Faculty of Medicine, Oita 879-5593, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea> Department of Anatomy, Biology and Medicine, Oita University Faculty of Medicine, Oita 879-5593</wicri:regionArea><wicri:noRegion>Oita 879-5593</wicri:noRegion></affiliation></author><author><name sortKey="Ritchlin, Christopher T" sort="Ritchlin, Christopher T" uniqKey="Ritchlin C" first="Christopher T." last="Ritchlin">Christopher T. Ritchlin</name><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Pytowski, Bronislaw" sort="Pytowski, Bronislaw" uniqKey="Pytowski B" first="Bronislaw" last="Pytowski">Bronislaw Pytowski</name><affiliation wicri:level="1"><nlm:aff id="A5"> Imclone Systems, New York City, NY 10014, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Imclone Systems, New York City, NY 10014</wicri:regionArea><wicri:noRegion>NY 10014</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Zhenping" sort="Zhu, Zhenping" uniqKey="Zhu Z" first="Zhenping" last="Zhu">Zhenping Zhu</name><affiliation wicri:level="1"><nlm:aff id="A5"> Imclone Systems, New York City, NY 10014, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Imclone Systems, New York City, NY 10014</wicri:regionArea><wicri:noRegion>NY 10014</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yong Jun" sort="Wang, Yong Jun" uniqKey="Wang Y" first="Yong-Jun" last="Wang">Yong-Jun Wang</name><affiliation wicri:level="1"><nlm:aff id="A6"> Shanghai University of Chinese Medicine, Shanghai, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Shanghai University of Chinese Medicine, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Schwarz, Edward M" sort="Schwarz, Edward M" uniqKey="Schwarz E" first="Edward M." last="Schwarz">Edward M. Schwarz</name><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Lianping" sort="Xing, Lianping" uniqKey="Xing L" first="Lianping" last="Xing">Lianping Xing</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19714652</idno><idno type="pmc">2810533</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810533</idno><idno type="RBID">PMC:2810533</idno><idno type="doi">10.1002/art.24764</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">002761</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002761</idno><idno type="wicri:Area/Pmc/Curation">002760</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002760</idno><idno type="wicri:Area/Pmc/Checkpoint">003703</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003703</idno><idno type="wicri:Area/Ncbi/Merge">003583</idno><idno type="wicri:Area/Ncbi/Curation">003583</idno><idno type="wicri:Area/Ncbi/Checkpoint">003583</idno><idno type="wicri:doubleKey">0004-3591:2009:Guo R:inhibition:of:lymphangiogenesis</idno><idno type="wicri:Area/Main/Merge">006311</idno><idno type="wicri:Area/Main/Curation">006236</idno><idno type="wicri:Area/Main/Exploration">006236</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inhibition of lymphangiogenesis and lymphatic drainage via VEGFR-3 blockade increases the severity of inflammation in chronic inflammatory arthritis</title><author><name sortKey="Guo, Ruolin" sort="Guo, Ruolin" uniqKey="Guo R" first="Ruolin" last="Guo">Ruolin Guo</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Quan" sort="Zhou, Quan" uniqKey="Zhou Q" first="Quan" last="Zhou">Quan Zhou</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Proulx, Steven T" sort="Proulx, Steven T" uniqKey="Proulx S" first="Steven T." last="Proulx">Steven T. Proulx</name><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Wood, Ronald" sort="Wood, Ronald" uniqKey="Wood R" first="Ronald" last="Wood">Ronald Wood</name><affiliation wicri:level="1"><nlm:aff id="A3"> Department of Urology, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Urology, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Ji, Rui Cheng" sort="Ji, Rui Cheng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name><affiliation wicri:level="1"><nlm:aff id="A4"> Department of Anatomy, Biology and Medicine, Oita University Faculty of Medicine, Oita 879-5593, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea> Department of Anatomy, Biology and Medicine, Oita University Faculty of Medicine, Oita 879-5593</wicri:regionArea><wicri:noRegion>Oita 879-5593</wicri:noRegion></affiliation></author><author><name sortKey="Ritchlin, Christopher T" sort="Ritchlin, Christopher T" uniqKey="Ritchlin C" first="Christopher T." last="Ritchlin">Christopher T. Ritchlin</name><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Pytowski, Bronislaw" sort="Pytowski, Bronislaw" uniqKey="Pytowski B" first="Bronislaw" last="Pytowski">Bronislaw Pytowski</name><affiliation wicri:level="1"><nlm:aff id="A5"> Imclone Systems, New York City, NY 10014, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Imclone Systems, New York City, NY 10014</wicri:regionArea><wicri:noRegion>NY 10014</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Zhenping" sort="Zhu, Zhenping" uniqKey="Zhu Z" first="Zhenping" last="Zhu">Zhenping Zhu</name><affiliation wicri:level="1"><nlm:aff id="A5"> Imclone Systems, New York City, NY 10014, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Imclone Systems, New York City, NY 10014</wicri:regionArea><wicri:noRegion>NY 10014</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yong Jun" sort="Wang, Yong Jun" uniqKey="Wang Y" first="Yong-Jun" last="Wang">Yong-Jun Wang</name><affiliation wicri:level="1"><nlm:aff id="A6"> Shanghai University of Chinese Medicine, Shanghai, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Shanghai University of Chinese Medicine, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Schwarz, Edward M" sort="Schwarz, Edward M" uniqKey="Schwarz E" first="Edward M." last="Schwarz">Edward M. Schwarz</name><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Lianping" sort="Xing, Lianping" uniqKey="Xing L" first="Lianping" last="Xing">Lianping Xing</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2"> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642</wicri:regionArea><wicri:noRegion>NY 14642</wicri:noRegion></affiliation></author></analytic><series><title level="j">Arthritis and rheumatism</title><idno type="ISSN">0004-3591</idno><idno type="eISSN">1529-0131</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Object</title><p id="P1">Investigation of the effect of lymphatic inhibition on joint and draining lymph node pathology during the course of arthritis progression in mice.</p></sec><sec sec-type="methods" id="S2"><title>Method</title><p id="P2">TNF transgenic (TNF-Tg) mice were used as a model of chronic inflammatory arthritis. Mice received contrast enhanced MRI to obtain ankle and knee joint synovial volumes and draining popliteal lymph node (PLN) volumes before and 8 weeks after treatment with VEGFR-3 or VEGFR-2 neutralizing antibodies, or isotype IgG. The animals were subjected to near-infrared lymphatic imaging to determine the effect of VEGFR-3 neutralization on lymph transport from paws to draining PLNs prior to sacrifice. Lymphatic vessel formation and morphology of joints and PLNs were examined by histology, immunohistochemistry, and RT-PCR.</p></sec><sec id="S3"><title>Results</title><p id="P3">Compared to IgG treatment, VEGFR-3 neutralizing antibody treatment significantly decreased the size of PLNs, the number of lymphatic vessels in joints and PLNs, the lymphatic drainage from paws to PLNs, and the number of VEGF-C expressing CD11b+ myeloid cells in PLNs. However, it increased the synovial volumes and inflammatory area in ankle and knee joints. VEGFR-2 neutralizing antibody, in contrast, inhibited both lymphangiogenesis and joint inflammation.</p></sec><sec id="S4"><title>Conclusion</title><p id="P4">Lymphangiogenesis and lymphatic drainage are reciprocally related to the severity of joint lesions during the development of chronic arthritis. Lymphatic drainage plays a beneficial role in controlling the progression of chronic inflammation.</p></sec></div></front></TEI><affiliations><list><country><li>Japon</li><li>République populaire de Chine</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Guo, Ruolin" sort="Guo, Ruolin" uniqKey="Guo R" first="Ruolin" last="Guo">Ruolin Guo</name></noRegion><name sortKey="Proulx, Steven T" sort="Proulx, Steven T" uniqKey="Proulx S" first="Steven T." last="Proulx">Steven T. Proulx</name><name sortKey="Pytowski, Bronislaw" sort="Pytowski, Bronislaw" uniqKey="Pytowski B" first="Bronislaw" last="Pytowski">Bronislaw Pytowski</name><name sortKey="Ritchlin, Christopher T" sort="Ritchlin, Christopher T" uniqKey="Ritchlin C" first="Christopher T." last="Ritchlin">Christopher T. Ritchlin</name><name sortKey="Schwarz, Edward M" sort="Schwarz, Edward M" uniqKey="Schwarz E" first="Edward M." last="Schwarz">Edward M. Schwarz</name><name sortKey="Wood, Ronald" sort="Wood, Ronald" uniqKey="Wood R" first="Ronald" last="Wood">Ronald Wood</name><name sortKey="Xing, Lianping" sort="Xing, Lianping" uniqKey="Xing L" first="Lianping" last="Xing">Lianping Xing</name><name sortKey="Xing, Lianping" sort="Xing, Lianping" uniqKey="Xing L" first="Lianping" last="Xing">Lianping Xing</name><name sortKey="Zhou, Quan" sort="Zhou, Quan" uniqKey="Zhou Q" first="Quan" last="Zhou">Quan Zhou</name><name sortKey="Zhu, Zhenping" sort="Zhu, Zhenping" uniqKey="Zhu Z" first="Zhenping" last="Zhu">Zhenping Zhu</name></country><country name="Japon"><noRegion><name sortKey="Ji, Rui Cheng" sort="Ji, Rui Cheng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name></noRegion></country><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Yong Jun" sort="Wang, Yong Jun" uniqKey="Wang Y" first="Yong-Jun" last="Wang">Yong-Jun Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006236 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006236 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:2810533
|texte= Inhibition of lymphangiogenesis and lymphatic drainage via VEGFR-3 blockade increases the severity of inflammation in chronic inflammatory arthritis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19714652" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |